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KMID : 0525720130180030152
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Journal of Chitin and Chitosan
2013 Volume.18 No. 3 p.152 ~ p.158
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Screening of Poly-γ-glutamic Acid (γ-PGA) Producing Bacteria from Environmental Soils
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Ju Wan-Taek
Song Yong-Su
Kim Young-Ju
Jung Woo-Jin
Park Ro-Dong
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Abstract
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Application of γ-PGA is limited due to the high cost associated with its production. Screening bacterial strains in order to find a more efficient producer is one of the platforms towards overcoming its limited usage. Over the 1500 colonies of bacteria were isolated from soil samples. From them, the 6 isolates, MJ80, CS4-29, HB-31, HB-31c, H1-19, and H1-07, were selected as candidates for γ-PGA production. Based on their morphological and biochemical properties and 16s rDNA sequences, MJ80 and CS4-29 were ultimately identified as Bacillus subtilis, H1-07 as B. thuringiensis, HB-31and HB-31c as B. anthracis, and H1-19 as B. sonorensis. The yield of γ-PGA ranged from 3.28 to 4.4%. It turned out that B. subtilis MJ80 and B. subtilis CS4-29 utilized both glutamic acid (GA) and soybean for γ-PGA production. B. thuringiensis H1-07 was identified as GA-dependent, while the other 3 strains were GA-independent. All strains produced high molecular weight γ-PGA over 200 kDa.
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KEYWORD
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γ-PGA, Bacillus subtilis, Glutamic acid, Soybean
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